She attended Perry High School in Perry, Ohio, graduating in 1987.
In 2000, she and Patty Rosborough won Ladies of Laughter, a multi-national competition for stand-up comics, hosted in the Northeastern United States.
In 2010, Pescatelli won Comedy Central's Standup Showdown. In 2004, she was a finalist on the second and third seasons of Last Comic Standing. She has performed on The Tonight Show with Jay Leno, The Talk, Rocco's Dinner Party, Comedy Central Presents, The World Stands Up, The Bonnie Hunt Show, Last Call with Carson Daly, Dennis Miller, and Comics Unleashed.
Pescatelli is a regular guest on The Bob & Tom Show in Indianapolis, and is a fixture on the comedy channels of Sirius XM Radio. On January 22, 2010, she was a guest on Opie and Anthony, an XM Satellite Radio talk show. She also appeared as a guest on Ron & Fez on SiriusXM.
A reality television show on WEtv, called A Standup Mother, related Pescatelli's life as a mother, wife, and comedian.
Her film appearances include minor roles in Made in Brooklyn, Single: A Documentary Film, and Everybody Wants to Be Italian.
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